ATAC-seq - csv upload

The Pluto ATAC-seq workflow

ATAC-seq is a technique used to determine chromatin accessibility on a genome wide scale.

1. Create Experiment

Start by choosing with project to associate your experiment with, the model organism for your experiment and the experiment type. Then press continue to proceed to your data uploads

2. Upload assay data

3. Upload sample annotations

Finally, you'll upload sample data for each of the samples in your dataset in a .csv file. Here is an example format for sample data from an ATAC-seq experiment. Pluto has templates available for download within the experiment set up dialogue.

4.  Start your analyses!

Next, you'll be directed to your experiment overview page where you can add notes to share with collaborators, see how many analyses you've run for that experiment and view uploaded sample data, assay data and the results from the ATAC-seq pipeline.

You can proceed to the Analysis tab by clicking "Analysis" at the top of the Overview page to start making plots. Press the "+Analysis" button to see what plots are available for your experiment.

The following plot types may be of interest for your ATAC-seq experiment