The Pluto ATAC-seq workflow
ATAC-seq is a technique used to determine chromatin accessibility on a genome wide scale.
1. Create Experiment
Start by choosing with project to associate your experiment with, the model organism for your experiment and the experiment type. Then press continue to proceed to your data uploads
2. Upload assay data
3. Upload sample annotations
Finally, you'll upload sample data for each of the samples in your dataset in a .csv file. Here is an example format for sample data from an ATAC-seq experiment. Pluto has templates available for download within the experiment set up dialogue.
4. Start your analyses!
You can proceed to the Analysis tab by clicking "Analysis" at the top of the Overview page to start making plots. Press the "+Analysis" button to see what plots are available for your experiment.
The following plot types may be of interest for your ATAC-seq experiment
- Differential Binding Analysis (Heatmap or Volcano plots)
- Functional Annotation Analysis
- Transcription Factor Enrichment Analysis
- Sample Correlation
- Coverage Analysis
- Summary Analysis
- Peak Analysis