Edit FASTQ files prior to analyzing an experiment in Pluto, or create a new experiment to re-process from scratch
Editing the FASTQ files on an experiment prior to analysis
When creating a sequencing-based experiment in Pluto, you can upload FASTQ files on the Assay Data upload step. Learn more about uploading FASTQs.
After you've finished uploading FASTQs, you'll click the blue "I'm done uploading files" button to lock in your FASTQ files for this experiment and the FASTQ upload box will be replaced by a green banner:
If you need to make changes, click the "Reopen FASTQ uploader" link to reopen the drag-and-drop upload area to add additional FASTQ files to your experiment.
Modifying FASTQ files after a pipeline has been run
If a pipeline has already been run successfully on an experiment, the FASTQ files for that experiment cannot be edited. You'll see a yellow warning message:
If you want to re-process these FASTQ files through a pipeline (and optionally add additional FASTQ files to process alongside them), you'll need to create a new copy of the experiment:
Copy over only the sample data and FASTQ files (NOT the assay data):
Then, on the newly copied experiment, click "Continue experiment setup" and navigate to the assay data upload step to either add additional FASTQs, or lock in the current FASTQs and proceed with running a pipeline.