Creating a Multi-omics experiment

Bring data from multiple Pluto experiments together into a unified multi-omics experiment for in-depth insights

Multi-omics refers to the integration of data from multiple molecular layers, such as genomics, transcriptomics, proteomics, and metabolomics, to provide a comprehensive and multi-dimensional view of biological systems. By combining these distinct yet complementary data types, multi-omics approaches allow for the investigation of complex cellular processes and the interplay between different molecular pathways. Unlike single-omics techniques, multi-omics provides a holistic perspective, enabling the exploration of how changes at the genetic, transcript, protein, and metabolite levels drive disease progression, treatment response, and therapeutic efficacy. Multi-omics has the potential to accelerate drug discovery and support the development of precision medicine strategies by revealing the full complexity of biological systems.

Creating a multi-omics experiment in Pluto is straightforward, requiring just a few clicks to set up your experiment and link relevant experiments together to facilitate a deeper, more comprehensive analysis.

Step 1: Create experiment

To create a multi-omics experiment, navigate to your Lab Space and create a new Multi-omics experiment. Be sure to give your experiment an informative name.

Note: Unlike other experiment types in Pluto, you do not need to select an organism for a Multi-omics experiment. Multi-omics experiments can support multiple organism types.

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Step 2: Select linked experiments

The multi-omics experiment in Pluto lets you analyze multiple experiments together. To add experiments to your multi-omics experiment, search for them by name or PLX ID.

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The experiments that you add to your multi-omics experiment are linked experiments. Once you've finished creating a multi-omics experiment, you'll be able to perform analyses on your linked experiments individually or integrate results across experiments.

Step 3: Perform analysis

After you finish creating your multi-omics experiment, you will land on the experiment's Canvas page. Each linked experiment is represented by an experiment card. From an experiment card, you can create a new analysis or select a previously created analysis for that linked experiment.

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You can also create a new analysis via the left-hand navigation toolbar. Here, you will be prompted to select which experiment you would like to create a new analysis from. You can select either your current, multi-omics experiment or one of your linked experiments.

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Step 4: Add more linked experiments

You can link additional experiments to your multi-omics experiment at any time after your multi-omics experiment has been created. Click on Data sources in the left-hand navigation toolbar, scroll to the bottom of the pop-out window, and click "+ Link an experiment". From there, search for the experiment you want to link by experiment name or PLX ID.

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Step 5: Organize your Canvas

As you are running analyses from your multi-omics experiment and linked experiments, be sure to utilize all the features that Canvas has to offer in order to tell your scientific story.