Differential expression analysis is a way to represent different levels of gene expression between conditions, allowing the user to easily find genes with a significant change in expression.
Set up your analysis
While viewing your experiment, click "+ Analysis" and select Differential expression analysis.
Select one or more Variables to group your samples by.
Select one of the resulting groups to be the Experimental Group and one group to be the Control Group.
Click the "Run Analysis" button to begin running differential expression analysis with the parameters you set above.
Customize your plots
Use the Plot tab to customize the title, color palette, and other characteristics of the plot that you created from the differential expression analysis.
Note: Analysis and plot parameters may vary from the examples above, depending on your organization's configuration. Contact your Pluto representative by email or using our in-app chat if you have any questions.