What is SortMeRNA?

Ribosomal RNA removal tool.

Written by Rani Powers, PhD


Takes as input raw RNA-seq files such as FASTQ or FASTA and one or more rRNA database files which it uses as a reference to remove rRNAs from the raw sequencing data. This is a long and memory intensive process but is highly important to ensure quality data analysis and further interpretation.


Evguenia Kopylova, Laurent Noé, Hélène Touzet, SortMeRNA: fast and accurate filtering of ribosomal RNAs in metatranscriptomic data, Bioinformatics, Volume 28, Issue 24, December 2012, Pages 3211–3217, https://doi.org/10.1093/bioinformatics/bts611