Read more about RSEM on the Pluto Blog.
RSEM is an accurate and user-friendly software package for quantifying gene and isoform abundances from single-end or paired-end RNA-Seq data. RSEM outputs abundance estimates, 95% credibility intervals, and visualization files and can be used with or without a reference genome.
Li, B., Dewey, C.N. RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome. BMC Bioinformatics 12, 323 (2011). https://doi.org/10.1186/1471-2105-12-323