Sequencing
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      1. Pluto Knowledge Base
      2. Reference: Analysis Terms & Methods
      3. Sequencing

      What is TPM?

      Transcripts per million fragments sequenced

      Written by Rani Powers, PhD


      Since RPKM is dependent on the length distribution of the transcripts, it isn’t a good measurement to compare between samples. To fix this, the number of counts mapped to a gene is only divided by the length-normalized reads instead of the total read count (click here for more information)