What is TPM?
Transcripts per million fragments sequenced
Written by Rani Powers, PhD
Since RPKM is dependent on the length distribution of the transcripts, it isn’t a good measurement to compare between samples. To fix this, the number of counts mapped to a gene is only divided by the length-normalized reads instead of the total read count (click here for more information)
![](https://help.pluto.bio/hubfs/Knowledge%20Base%20Import/downloads.intercomcdn.comio33454474867ee84894de1575d0a9d8481image.png)