What is RPKM?
Reads per kilobase of transcript per million reads
Written by Rani Powers, PhD
A unit of measurement based on the number of counts mapped to a gene scaled by both the total number of counts and the length of the fragment.
Recommendations for use: gene count comparisons between genes within a sample; NOT for between sample comparisons or DE analysis.
![](https://help.pluto.bio/hubfs/Knowledge%20Base%20Import/downloads.intercomcdn.comio33454454289d1eab0b13067fbcb96b216image.png)