Sequencing
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      1. Pluto Knowledge Base
      2. Reference: Analysis Terms & Methods
      3. Sequencing

      What is RPKM?

      Reads per kilobase of transcript per million reads

      Written by Rani Powers, PhD


      A unit of measurement based on the number of counts mapped to a gene scaled by both the total number of counts and the length of the fragment.


      Recommendations for use: gene count comparisons between genes within a sample; NOT for between sample comparisons or DE analysis.